BAC Recombineering Disclaimer

Most bacterial recombinations can be efficiently performed using homology arms as short as 50 bases.  Since this avoids the need for PCR amplification we typically synthesize these homology arms as a pair of complementary oligonucleotides.  Rarely, a particular BAC sequence is refractory to efficient recombination, as evidenced by poor colony yield and/or failure to obtain desired recombinants upon clone screening.  Unfortunately it is not possible to predict “difficult” recombination targets before this is empirically discovered.  The investigator will be responsible for labor and supply costs involved in vector construction regardless of outcome. Core service charges are applied the month that construction is initiated.  If recombination difficulty arises, Dr. Mortlock will notify the investigator promptly so potential changes in strategy can be discussed and implemented.  For example, increasing the length of the homology arms can sometimes improve recombination efficiency, though this does not always solve the problem.