pCMV4.GKL1

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Last modified on August 17, 2010 at 3:24 PM by Rama Gangula

Summary description:

 

This plasmid is used for expressing the liver/hepatic isoform of glucokinase in eukaryotic cell lines.  It contains a rat glucokinase cDNA (hepatic isoform) fragment that was generated by PCR amplification, then cloned into pCMV4, a plasmid that drives expression from using a cytomegalovirus promoter, a translational augmenter from alfalfa mosaic virus and 3’ splice and polyadenylation signals from human growth hormone gene.   

 

Resource Data
Vector Map Image ybeZljP5.png
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Backbone Vector pCMV4
Construct Size 6,279 bp (approximate)
Source Laboratory: Mark Magnuson
Made by: Tod Martin
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Stock Date December 19, 2008
Genbank File pCMV4.GKL1.gb
Location Not Available