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PDFpRIP.Cre.GH
This plasmid vector is being viewed within the MagLab Stocks / Plasmids collection. View other collections this resource is shared to.
Summary description:
This plasmid contains an insulin-cre-hGH fusion gene that was made as follows. (1) The 1.2 kb NLSKOZCre gene from pmL78 was subcloned into pBSIIKS at Sal I and EcoRI. (2) The 0.7 kb rat insulin promoter 2 gene (RIP) was subcloned from pSPRIP into the Kpn I and Xho I sites. (3) The 2.1 kb growth hormone gene from pOGH was cut at BamHI and EcoRI, blunted, and Spe I sites introduced on either end; this GH gene fragment was then subcloned into the Spe I site of the clone from step 2. (4) A small Sal I/Sal I polylinker fragment between RIP and Cre was deleted. (5) The final construct (pBS/RIP-Cre-GH) was cut at Kpn I and Not I and used for microinjection.
This plasmid was used to generate RIP(Ins2)-Cre transgenic mice.
| Resource Data | |
|---|---|
| Vector Map Image |
· Click on image or here to view larger size. · Click here to download native resolution image. |
| Backbone Vector | pBluescript II KS |
| Construct Size | 6,936 bp (approximate) |
| Source |
Laboratory: Mark Magnuson Made by: Yuejin Chen |
| Bacterial Stock | No |
| Storage Temperature | -20°C |
| Stock Concentration | 0.1 µg/µL |
| Stock Date | December 23, 2008 |
| Genbank File | pRIP-Cre-GH.gb |
| Location | Not Available |
