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PDFPreparation of mouse ES cells for karyotyping
This protocol document is being viewed within the TMESCSR Protocols - Lab Only collection. View other collections this resource is shared to.
Last modified on July 2, 2010 at 1:15 PM by Jill Lindner
Summary description:
Summary description:
This protocol may be used to prepare mouse ES cells for karyotyping.
Reagents. supplies, and equipment:
- cES cell media
- 60 mm tissue culture dish with MEFs
Procedure:
- Please notify the TMESCSR that you have mES cells that you would like to have karyotyped. The Resource will schedule and coordinate a time for cells to be delivered for karyotyping.
- E-mail the names and clone numbers of the cell lines requested for karyotyping to Jake Stein (jake.stein@vanderbilt.edu) and Jennifer Skelton (jennifer.skelton@vanderbilt.edu). Label the culture dishes with the same names.
- On a 60 mm culture dish, plate cells on a layer of MEFs.
- Colonies need to be at least medium in size and 50% to 90% confluent for karyotyping. This is usually about two days of growth.
- On the day dishes are being handed off to the TMESCSR for karyotyping, feed the plate(s) with cES before 10:00 AM and communicate to the TMESCSR personnel what time they were fed.
- Bring plates for karyotyping to the TMESCSR in 9410 MRB IV by 11:30 AM.
Source: TMESCSR 4/10
