pGST.GKB1

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Last modified on August 20, 2010 at 9:57 AM by Rama Gangula

Summary description:

The expression vector pGEX-3X was used for eukaryotic expression of a glucokinase (gk) cDNA fragment from pGK.B1.  A rat gk cDNA fragment with an upstream BamHI site and a downstream EcoRI site was generated by linker-primer PCR and ligated into pGEX-3X.  The plasmid is referred to as both pGST.GKB1 and pGEX.GKB1.

Resource Data
Vector Map Image PqQ7J0Fe.png
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Backbone Vector pGEX-3X
Construct Size 6,340 bp (approximate)
Source Laboratory: Mark Magnuson
Made by: Kathy Shelton
Bacterial Stock No
Storage Temperature -20°C
Stock Concentration 0.1 µg/µL
Stock Date December 23, 2008
Genbank File GST.GKB1.gb
Location Not Available